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Overview
The Advanced Microscopy (AM) Unit provides expertise and tools for the analysis of the structural and molecular organization of both live/fixed cells and tissues, and time-lapse investigations.
The service allows access to state-of-the-art optical microscopy instruments including transmitted light, fluorescence and confocal instruments, providing training and support for the correct use of the tools.
This service is intended for both members of the University of Piemonte Orientale and external user and companies.
Services
We offer
• Support in experimental design
• Training of new users
• Live imaging/time-lapse acquisition and analysis
• Multi-color image acquisition and 3D rendering on images acquired by the confocal microscope
• Support for image processing and quantitative analysis of data
Infrastructure
To reserve these instruments/services, it is necessary to be registered with the "Agendo" centralized management system.
To register, fill in the following Registration Form
If you are already registered, log in to the system https://upo.agendoscience.com/
Equipment
Leica THUNDER Imager 3D Live Cell
Fully motorized inverted fluorescence microscope DMi8
o Autofocus
o Objectives: 5x/20x/63x(oil)
o Filter Cubes: DAPI/FITC/TxR/Cy5
Camera: Leica DFC9000 GTC
Cage incubator
Leica TCS SP8 LIGHTNING confocal laser scanning microscope
Fully motorized inverted fluorescence microscope DMi8 CS Bino
o Autofocus
o Objectives: 10x/20x/40x(oil)/63x(oil)
o Filter Cubes: DAPI LP/FITC LP/RHOD LP
o DIC system accessories for 40x & 63x objectives
Stage incubator
Laser Lines: 405/458/476/488/496/514/561/633 nm
4 detectors:
o 2 PMT
o 2 HyD GaAsP
EVOS Floid imaging system
Leica STELLARIS SP8 DIVE
Fully motorized upright fluorescence microscope DM6 CFS flex
Objectives:
o HC FLUOTAR L 25x/0.95 W VISIR (water immersion)
o HCX APO L 40X/0.80 W UVI (water immersion)
o HCX PL APO 40x/1.25-0.75 Oil (oil immersion)
o HCX PL APO 63x/1.40-0.6 Oil (oil immersion)
SP8 CLSM equipped with white light laser (470-650nm) and a LED Laser 405 nm
STELLARIS DIVE 2-photon LSM equipped with IR Laser Mai Tai HP, 690-1040nm
3 hybrid HyD GaAsP detectors and 3 PMTs
4-Tune module
FALCON-FLIM module
LAS X software.
How to access facility services
Users should contact the Facility manger (marco.corazzari@uniupo.it) and request a quote by filling out the order form with detailed information about the service required.
Price list 2022
Monitoring of services
by Agendo system: https://upo.agendoscience.com/
Unit Members
Simone Reano - technician
Contacts
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