Metabolomics & Proteomics

OverviewThe Unit Metabolomics & Proteomics (MP) is equipped with state of-art instrumentation to provide comprehensive analyses of proteins, small molecules and lipids from different matrices (e.g.: cells, biological fluids, environment, food …).

The laboratory is centered around two high-resolution mass spectrometer coupled to liquid chromatography and one bidimensional gas chromatography mass spectrometry.

If needed, the staff can develop new customized analytical methods and can perform data analysis, pathway analysis and omics data integration.

Instrumentations

The Unit is equipped with:

·      Thermo Scientific Q Exactive Plus Mass Spectrometer + Vanquish UHPLC Systems

·      Sciex Triple TOF 5600 plus + micro LC 200 eksigent or easy nanoLC Thermo

·      LECO Pegasus BT 4D GCxGC-TOF (SPME and HS)

·      Ultimate 3000 biocompatible + DAD

·      Gel-proteomics (SERVA HPE blue Horizon, IEF Bio-Rad, Chemidoc)

·      FastPrep 24, Ultraturrax, point sonicator

·      Positive pressure-96 for SPE

·      Liquid handling for ELISA

·      Bioinformatic analysis: IPA Ingenuity Pathway Analysis, Cytoscape, STRING, PANTHER, Metaboanalyst, etc..

Services

To reserve these instruments/services, it is necessary to be registered with the "Agendo" centralized management system.

To register, fill in the following Registration Form

If you are already registered, log in to the system https://upo.agendoscience.com/

The Proteomics and Metabolomics Lab offers a range of different services:

  Proteomics

·       Global quantification of proteomes (label-free and label-based)

·       Biomarkers discovery (immunodepletion of most abundant proteins)

·       Immunoprecipitation analysis

·       Identification and site-localization of selected post-translational modifications

·       Identification of gel-separated, Coomassie-stained and WB proteins

·       Protein fractionation with LC

·       Data analysis for all of the services listed above

·       Storage of raw and processed MS data in publicly available repositories

  Metabolomics

·       Global untargeted metabolomic profiling

·       Targeted metabolomic profiling

·       Lipidomic analysis

·       Metabolite and lipid identification

·       Comparative metabolic profiling (UPLC-MS-MS and GCxGC-MS)

·       High throughput data analysis (including basic statistics)

  Prices