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Metagenomics

Overview

The Metagenomics (META) Unit identifies the quality and quantity of the microorganisms present in an environment (microbiota) through the analysis of their genomes (microbiome) and transcriptomes (meta-transcriptomics).

The Unit works in synergy with other units to provide a broad range of analysis starting from “out-of-the-box” first-level profiling of microbiota to high level study of meta-genomes/meta-transcriptomess to highlight the functional capacity of the organisms.

Services

To reserve these services, it is necessary to be registered with the "Agendo" centralized management system.
To register, fill in the following form 
https://forms.gle/3judN7b5j2UcoFicA.

If you are already registered, log in to the system https://upo.agendo.science/

Currently available services comprise the sequencing of the 16S rDNA gene to profile the microbiome community in a complex biological sample.

16S rDNA regions: V1-V2-V3 or V3-V4-V6 

The service comprises:

DNA extraction, DNA Quality Control, Library preparation, Sequening, Data analysis and Report.

The reporting  consists on:

  • Raw results: text file with abundances for any taxonomic level
  • Standard report: pdf file summarizing sequences statistics, alpha diversity, classification of top 10 abundances for each taxonomic level (table and chart), quality information about unassigned sequences.
  • Custom report: a custom pdf file with other information can be provided. It is also possible to add a personal logo and footer to the document.

For specific purposes, bacteria identification can be performed by MALDI TOF analysis (Biotyper app).

 Costs:

 

UPO user

External user

Standard report

85 euro/sample

110 euro/sample

Custom report

90 euro/sample

120 euro/sample

 (costs may vary for high number of samples > 80)

 

Samples comparison

Data can also be analyzed to compare samples collected in different conditions or at different timepoints.
Typical analysis consists on a beta diversity, PCA and clustering analysis, however exact report will be discussed depending on the need of the project.

Costs:

 

UPO user

External user

Sample comparison

50 euro/comparison

 200 euro/comparison

Note: the price does not depend on number of samples.

 Service traceability

 Analysis and result layouts are provided by proprietary software.

 Contacts

flavio.mignone@uniupo.it