SERVICES‎ > ‎

Metabolomics & Proteomics

Overview

The Unit Metabolomics & Proteomics (MP) is equipped with state of-art instrumentation to provide comprehensive analyses of proteins, small molecules and lipids from different matrices (e.g.: cells, biological fluids, environment, food …).
The laboratory is centered around two high-resolution mass spectrometer coupled to liquid chromatography and one bidimensional gas chromatography mass spectrometry.
If needed, the staff can develop new customized analytical methods and can perform data analysis, pathway analysis and omics data integration.

Instrumentations

The Unit is equipped with:

·      Thermo Scientific Q Exactive Plus Mass Spectrometer + Vanquish UHPLC Systems

·      Sciex Triple TOF 5600 plus + micro LC 200 eksigent or easy nanoLC Thermo

·      LECO Pegasus BT 4D GCxGC-TOF (SPME and HS)

·      Ultimate 3000 biocompatible + DAD

·      Gel-proteomics (SERVA HPE blue Horizon, IEF Biorad, Chemidoc)

·      FastPrep 24, Ultraturrax, point sonicator

·      Positive pressure-96 for SPE

·      Liquid handling for ELISA

·      Bioinformatic analysis: IPA Ingenuity Pathway Analysis, Cytoscape, STRING, PANTHER, Metaboanalyst, etc..

Services

The Proteomics and Metabolomics Lab offers a range of different services:

 

 Proteomics

·       Global quantification of proteomes (label-free and label-based)

·       Biomarkers discovery (immunodepletion of most abundant proteins)

·       Immunoprecipitation analysis

·       Identification and site-localization of selected post-translational modifications

·       Identification of gel-separated, Coomassie-stained and WB proteins

·       Protein fractionation with LC

·       Data analysis for all of the services listed above

·       Storage of raw and processed MS data in publicly available repositories

 

 Metabolomics

·       Global untargeted metabolomic profiling

·       Targeted metabolomic profiling

·       Lipidomic analysis

·       Metabolite and lipid identification

·       Comparative metabolic profiling (UPLC-MS-MS and GCxGC-MS)

·       High throughput data analysis (including basic statistics)

  

Prices

Services

UPO
(euro)

external
(euro)

Protein identification from spot/gel (for sample)

60

80

Protein identification complex sample (1 microLC run)

160

200

Serum semi-quantitative analysis label-free (included immunodepletion) with microLC (for sample)

400

500

Semi-quantitative analysis label-free (couple)

750

900

Untargeted metabolomics (LC-HRMS) (1 run)

250

350

Untargeted lipidomics (LC-HRMS) (1 run)

200

300

Untargeted metabolomics (GC×GC-TOFMS) (liquid injection, 1 run)

200

300

Sample preparation (based on sample type and analysis)

from 30

from 45

Bioinformatic analysis

50/hour

100/hour

In general the cost depends very heavily on type of sample, number of samples and on targets.
Reported prices are indicative, for your particular study please contact: marcello.manfredi@uniupo.it

The laboratory guides investigators through the experimental design and statistical considerations of their studies.

Service traceability

Web based layout reporting analyses, data, and instrument’s metadata contents.

Training

Training on basic procedures will be scheduled twice at year.

Unit Members

 

Contatti

marcello.manfredi@uniupo.it